CHEM
584
Adv Biochemistry Methods 1
Hours
3.0 Credit, 1 Lecture, 6 Lab
Semester
Fall
Introduction to current biochemical research methods, techniques, and instrumentation directed toward the isolation and characterization of DNA, RNA, and proteins including, DNA cloning, protein expression, enzyme kinetics, and nucleic acid and protein ligand interactions.
Design and execute experiments with nucleic acids applicable to prokaryotic systems
Apply nucleic acid techniques to isolate, purify, characterize, manipulate, amplify and express DNA and/or RNA in prokaryotic systems.
Design and execute experiments with proteins derived from gene expression in prokaryotes
Characterize and manipulate proteins obtained from gene expression in prokaryotic organisms.
Clearly communicate research approaches and data
Communicate in written and verbal forms the methods and results of experimentation. Provide sufficient explanation of experimental techniques and approaches to enable others to repeat experiments and verify results.
Use of existing resources to address research questions
Utilize available resources (e.g., online, manufacturer's protocols) to plan, execute, and troubleshoot experimental approaches.