Adv Biochemistry Methods 1

Hours

3.0 Credit, 1 Lecture, 6 Lab

Semester

Fall

Introduction to current biochemical research methods, techniques, and instrumentation directed toward the isolation and characterization of DNA, RNA, and proteins including, DNA cloning, protein expression, enzyme kinetics, and nucleic acid and protein ligand interactions.

Design and execute experiments with nucleic acids applicable to prokaryotic systems

Apply nucleic acid techniques to isolate, purify, characterize, manipulate, amplify and express DNA and/or RNA in prokaryotic systems.

Design and execute experiments with proteins derived from gene expression in prokaryotes

Characterize and manipulate proteins obtained from gene expression in prokaryotic organisms.

Clearly communicate research approaches and data

Communicate in written and verbal forms the methods and results of experimentation. Provide sufficient explanation of experimental techniques and approaches to enable others to repeat experiments and verify results.

Use of existing resources to address research questions

Utilize available resources (e.g., online, manufacturer's protocols) to plan, execute, and troubleshoot experimental approaches.